At the southern margin of permafrost in North America, climate change causes widespread permafrost thaw. In boreal lowlands, thawing forested permafrost peat plateaus (‘forest’) lead to expansion of permafrost-free wetlands (‘wetland’). Expanding wetland area with saturated and warmer organic soils is expected to increase landscape methane (CH4) emissions. Here, we quantify the thaw-induced increase in CH4 emissions for a boreal forest-wetland landscape in the southern Taiga Plains, Canada, and evaluate its impact on net radiative forcing relative to potential long-term net carbon dioxide (CO2) exchange. Using nested wetland and landscape eddy covariance net CH4 flux measurements in combination with flux footprint modeling, we find that landscape CH4 emissions increase with increasing wetland-to-forest ratio. Landscape CH4emissions are most sensitive to this ratio during peak emission periods, when wetland soils are up to 10 °C warmer than forest soils. The cumulative growing season (May–October) wetland CH4emission of ~13 g CH4 m−2 is the dominating contribution to the landscape CH4 emission of ~7 g CH4 m−2. In contrast, forest contributions to landscape CH4 emissions appear to be negligible. The rapid wetland expansion of 0.26 ± 0.05% yr−1 in this region causes an estimated growing season increase of 0.034 ± 0.007 g CH4 m−2 yr−1 in landscape CH4 emissions. A long-term net CO2 uptake of >200 g CO2 m−2 yr−1 is required to offset the positive radiative forcing of increasing CH4emissions until the end of the 21st century as indicated by an atmospheric CH4 and CO2concentration model. However, long-term apparent carbon accumulation rates in similar boreal forest-wetland landscapes and eddy covariance landscape net CO2 flux measurements suggest a long-term net CO2 uptake between 49 and 157 g CO2 m−2 yr−1. Thus, thaw-induced CH4 emission increases likely exert a positive net radiative greenhouse gas forcing through the 21st century.