@article {557,
	title = {Effects of continuous copper exposure and calcium on the olfactory response of fathead minnows},
	journal = {Environmental Science and Technology},
	volume = {46},
	year = {2012},
	note = {n/a},
	pages = {9019-9026},
	abstract = {The current gill-based Biotic Ligand Model (gbBLM) is an acute-toxicity model used to predict site-specific safe copper (Cu) concentrations. Recent effort to develop a chronic BLM has focused on the olfactory epithelium. To further this effort, the current study looked at the effect of varying Cu concentration and exposure duration on Cu-induced olfactory dysfunction, and whether calcium (Ca) protected against Cu-induced impairment as it does at the gill. Fathead minnows (Pimephales promelas) were treated with five Cu concentrations for varying exposure durations in hard and soft water. A neurophysiological technique, electro-olfactography (EOG), was employed to determine the level of olfactory dysfunction. At the low, ecologically relevant Cu concentrations tested there was significant inhibition of EOG function; however, over time there was at least a partial recovery of olfactory function, despite the continuous Cu exposure. Calcium did not appear to protect against Cu-induced olfactory dysfunction; and even alone, Ca appeared to interfere with the olfactory response to the amino acid L-arginine. Safe copper concentrations as predicted by the gbBLM, chemosensory-based BLMs, the USEPA BLM, and hardness-adjustment equations based on the exposure waters were not entirely protective against olfactory dysfunction.},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=22794350},
	author = {Dew, WA and Wood, CM and Pyle, GG}
}
