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Publications

In Press, 2021
Czekay DP, Kothe U. H/ACA small Ribonucleoproteins: structural and functional comparison between archaea and eukaryotes. Frontiers in Microbiology [Internet]. In Press, 2021. Publisher's Version
Porat J, Kothe U, Bayfield MA. Revisiting tRNA chaperones: New players in an ancient game. RNA [Internet]. In Press, 2021. Publisher's Version
2020
Keffer-Wilkes LC, Soon EF, Kothe U. The methyltransferase TrmA facilitates tRNA folding through interaction with its RNA-binding domain. Nucleic Acids Research [Internet]. 2020;48(14):7981 - 7990. Publisher's Version
Roberts L, Kothe U, Wieden H-J. Parenting researchers-an invisible divide. EMBO Reports [Internet]. 2020;21(8). Publisher's Version
Schultz SK-L, Kothe U. tRNA elbow modifications affect the tRNA pseudouridine synthase TruB and the methyltransferase TrmA. RNA [Internet]. 2020;26(9):1131 - 1142. Publisher's Version
Vos TJ, Kothe U. snR30/U17 Small Nucleolar Ribonucleoprotein: A Critical Player during Ribosome Biogenesis. Cells [Internet]. 2020;9(10). Publisher's Version
Thakor N, Kothe U, Wieden H-J, Patel T. Proceedings of the 14th annual RiboWest conference: perspectives and outcome. Biochemistry and Cell Biology [Internet]. 2020;98(1):vii-ix. Publisher's Version
2019
Kelly EK, Czekay DP, Kothe U. Base pairing interactions between substrate RNA and H/ACA guide RNA modulate the kinetics of pseudouridylation, but not the affinity of substrate binding by H/ACA small nucleolar Ribonucleoproteins. RNA. 2019.
2018
Caton EA, Kelly EK, Kamalampeta R, Kothe U. Efficient RNA pseudouridylation by eukaryotic H/ACA ribonucleoproteins requires high affinity binding and correct positioning of guide RNA. Nucleic Acids Research [Internet]. 2018;46(2):905-916. Publisher's Version
Tillault A-S, Schultz SK, Wieden H-J, Kothe U. Molecular Determinants for 23S rRNA Recognition and Modification by the E. coli Pseudouridine Synthase RluE. Journal of Molecular Biology [Internet]. 2018;430(9):1284-1294. Publisher's Version
2017
Rintala-Dempsey AC, Kothe U. Eukaryotic stand-alone pseudouridine synthases - RNA modifying enzymes and emerging regulators of gene expression?. RNA Biology [Internet]. 2017;14(9):1185-1196. Publisher's Version
2016
Keffer-Wilkes L, Veerareddygari GR, Kothe U. RNA modification enzyme TruB is a tRNA chaperone. PNAS. 2016;113:14306 – 14311.
2015
Tillault AS, Fourmann JB, Loegler C, Wieden HJ, Kothe U, Charpentier B. Contribution of two conserved histidines to the dual activity of archaeal RNA guide-dependent and -independent pseudouridine synthase Cbf5. RNA. 2015;accepted.
2014
Friedt J. An arginine-aspartate network in the active site of bacterial TruB is critical for catalyzing pseudouridine formation. Nucleic Acids Research. 2014;42(6):3857-3870.
2013
Kamalampeta R, et al. tRNA binding, positioning and modification by the pseudouridine synthase Pus10. JMB. 2013;425(25):3863-3974.
2012
Kamalampeta R, Kothe U. Archaeal proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA. Scientific Reports. 2012;2(663):DOI: 10.1038/srep00663.
Kothe U. New frontiers in studying pseudouridine formation in RNA. Cell News. 2012;2:48-56. kothecellnews_02_2012.pdf
2011
Wright JR, Keffer-Wilkes LC, Dobing SR, Kothe U. Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA and RluA reveals uniformly slow catalysis. RNA. 2011;17:2074-2084.
2010
Kothe U. Recent Progress on Understanding Ribosomal Protein Synthesis. In: Comprehensive Natural Products II Chemistry and Biology, Section Amino Acids, Peptides and Proteins. Oxford: Elsevier; 2010.
2007
Kothe U, Rodnina MV. Codon reading by tRNAAla with modified uridine in the wobble position. Molecular Cell. 2007;25(1):167-174.
2006
Kothe U, Rodnina MV. Delayed release of inorganic phosphate from elongation factor Tu following GTP hydrolysis on the ribosome. Biochemistry. 2006;45:12767-12774.
Kothe U, Paleskava L, Konevega AL, Rodnina MV. Single-step purification of specific tRNAs by hydrophobic tagging. Analtyical Biochemistry. 2006;356:148-150.
2005
Savelsbergh A, Mohr D, Kothe U, Wintermeyer W, Rodnina MV. Control of Phosphate Release from Elongation Factor G by Ribosomal Protein L7/12. EMBO Journal. 2005;24(24):4316-4323.
Diaconu M, Kothe U, Schluenzen F, Fischer N, Harms J, Tonevitski AG, Stark H, Rodnina MV, Wahl MC. Structural basis for the Function of the Ribosomal L7/12 Stalk in Factor Binding and Activation of GTP Hydrolysis. Cell. 2005;121(7):991-1004.
2004
Kothe U, Wieden H-J, Mohr D, Rodnina MV. Interaction of Helix D of Elongation Factor Tu with Helices 4 and 5 of Protein L7/12 on the Ribosome. Journal of Molecular Biology. 2004;336:1011-1021.